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Description
Human PFKFB2 (6-phosphofructo-2-kinase, Fructose-2, 6-biphosphatase 2) ELISA KitThe Human (PFKFB2) 6 phosphofructo 2 kinase, Fructose 2, 6 biphosphatase 2 ELISA Kit measures 6 phosphofructo 2 kinase fructose 2,6 biphosphatase 2 in samples. The plate has been pre coated with Human PFKFB2 antibody. PFKFB2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PFKFB2 Antibody is added and binds to PFKFB2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated
The Human (PFKFB2) 6-phosphofructo-2-kinase, Fructose-2, 6-biphosphatase 2 ELISA Kit measures 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 in samples. The plate has been pre-coated with Human PFKFB2 antibody. PFKFB2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PFKFB2 Antibody is added and binds to PFKFB2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PFKFB2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PFKFB2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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