DHT ELISA Kit

DHT ELISA KitThe Dihydrotestosterone (DHT) ELISA kit is a competitive ELISA to measure DHT in serum. Competition occurs between DHT present in calibrators, controls, specimen samples and an enzyme labelled antigen (HRP conjugate) for a limited number of anti DHT antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue colored product that

The Dihydrotestosterone (DHT) ELISA kit is a competitive ELISA to measure DHT in serum. Competition occurs between DHT present in calibrators, controls, specimen samples and an enzyme-labelled antigen (HRP conjugate) for a limited number of anti-DHT antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-colored product that is inversely proportional to the amount of DHT present. Following an incubation, the enzymatic reaction is terminated by the addition of the stopping solution, converting the color from blue to yellow. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the amount of DHT in specimen samples and controls can be directly read.