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Description
Human VEGF ELISA KitProduct Specification Species Reactivity Human Theory This kit adopts double antibody sandwich enzyme linked immunosorbent detection technology. Specific anti human VEGF antibodies were pre coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the VEGF present in the sample binds to the solid phase antibody and the
Product Specification
| Species Reactivity | Human | |||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human VEGF antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the VEGF present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference corrected wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Synonym | MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGF, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor | |||||||||||||||||||||||||||
| Composition |
Please use within the expiration date of the kit
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| Background | Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor, is an effective regulator of angiogenesis and angiogenesis in fetus and adults. Vascular endothelial growth factor belongs to the PDGF family, a family of proteins characterized by the presence of eight conserved cystine residues at the structure of the cystine knot and the dimer bound by antiparallel disulfide bonds. After alternative cleavage and sequence length of amino acids, humans express different isoforms, including: VEGF121, VEGF145, VEGF165, VEGF183, VEGF189 and VEGF206, among others. Among them, VEGF165 is the highest expression isoform, followed by VEGF121 and VEGF189. With the exception of VEGF121, the other isoforms contain a basic heparin-binding region and do not diffuse freely. Human VEGF165 has 88% homology to the corresponding protein amino acid sequences of mouse and rat. VEGF is expressed in various cells and tissues, including skeletal muscle cells and cardiomyocytes, hepatocytes, osteoblasts, neutrophils, macrophages, keratinocytes, brown adipocytes, CD34 + stem cells, endothelial cells, fibroblasts, vascular smooth muscle cells, and the like. The expression of VEGF is induced by hypoxia and cytokines, including IL-1, IL-6, IL-8, oncostatin M and tumor necrosis factor alpha. The expression level of VEGF is also different during development and in adults. The dimer of VEGF binds to two related tyrosine kinase receptors, namely VEGFR1 (also called Flt-1) and VEGFR2 (Flk-1/KDR). VEGF can induce both homodimerization and autophosphorylation of the latter. These receptors have seven extracellular immunoglobulin domains and one intracellular separate tyrosine receptor domain. Both vascular endothelial cells and some other non-endothelial cells express VEGF receptors. Although VEGF has the highest affinity with VEGFR1, VEGFR2 is the main factor regulating angiogenesis of VEGF. VEGF165 also binds to the semaporin receptor, Neuropilin-1, thereby promoting complex formation with VEGFR2. VEGF is known for its involvement in angiogenesis. During embryonic development, VEGF regulates the proliferation, migration and survival of endothelial cells, and thus regulates the density and volume of blood vessels. But it doesn't work on the pattern of vascularization. VEGF promotes bone formation through the recruitment of osteoblasts and chondrocytes, and it is also a monocyte chemokine. Postpartum, VEGF maintains the integrity of vascular endothelial cells and is an effective mitogen for large/small vascular endothelial cells. In adults, VEGF plays a role mainly in wound repair and the female reproductive cycle. In diseased tissues, VEGF promotes vascular permeability. Therefore, VEGF is involved in the metastatic process of tumors through extravasation and tumor angiogenesis. Various therapeutic strategies aimed at blocking VEGF activity are being used to control tumor angiogenesis induced by VEGF. The level of VEGF circulating in the body is related to the extent of autoimmune diseases (such as rheumatoid arthritis, multiple sclerosis, systemic lupus erythematosus, etc.). |
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| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
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| Storage Temp. | Kit unopened, stored at 2-8 °C. | |||||||||||||||||||||||||||
| Test Range | 31.3pg/mL-2000pg/mL |
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4.9 ★★★★★
Based on 30 reviews
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Product Reviews
★★★★★ 4
Fluffy and Nice Omegaverse
Format: Kindle
… this would have made 5 stars but for 2 reasons.
A.) there were quite a few typos; misspelled words, missing quotations, “the his” mistakes, and various signs that maybe a proofread would do good.
B.) the writing was quite textbook. Late blooming omega is struggling with her new self, finds a absurdly wealthy pack of alphas, every thing is almost insta-love but she resists, then decides to love herself and let everyone be happy.
Rian was my favourite (obviously the author’s favourite too because he got the most page time) but I wish we could see more of his CEO side? He went to work maybe ONCE the entire time. Gray was supposed to be the “growly one” but he turned out to be puppy dog. Lucas was a genius brainiac doctor - but also super alpha with an aggressive hindbrain with a breeding k*nk?? And then there was no actual “breeding”??
Spice 3/5 - normally omegaverse books are super high on messy smut but this was tamer.
Romance 3/5 - insta-love that was then resisted because of personal hangup’s
Plot 2/5 - weird paced head hopping, showing the same scene from different POV’s that made me feel like it was 2 steps backward, 1 step forward.
Humour 4/5 - there were a dozen lines that genuinely made me chuckle out loud
Would have been five stars but the lack of proofreading and the predictable plot made me unable to get up to ADORED IT level - four stars is still and official ENJOYED IT, y’all. This isn’t a bad rating. The “Club Heat” has intriguing possibilities so I’m going to give the second one a shot.
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Reviewed in the United States on March 31, 2023
★★★★★ 5
Just read it. You won’t regret it.
Format: Hardcover
This book completely blew me away. I loved every single character—not just the FMC and MMC, but the side characters too, which is rare. In a first book, side characters can feel flat while the focus is on world-building, but that definitely wasn’t the case here.
The romantic tension between the FMC and MMC was so good—seriously addictive. The plot itself felt pretty straightforward at first, and the world-building was easy to follow. It even gave me Heartless Hunter / Wolf King vibes… until the last 20 pages.
And wow—those final pages. I usually spot plot twists coming, and I thought this one was going to be predictable, but I was completely wrong. That ending genuinely shocked me—I haven’t had that reaction to a book in a long time.
I’m already counting down to book two (and that teaser chapter? Absolutely brutal).
Just read it. You won’t regret it.
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Reviewed in the United States on May 4, 2026
★★★★★ 4
4 Stars for Now!
Format: Kindle
I DEVOURED this book. I was in a huge slump after reading a horrible series right before this but after 3 pages of this book, I was hooked. I read the whole book in 2 days. I think the author did an amazing job at writing an engaging story with an amazing twist at the end. I know many readers feel betrayed by the twist and find the narrator to be unreliable but I feel that the twist was done in a great unsuspecting way. Though I do feel betrayed, I think there is so much room for redemption in the second book!
The author also did an amazing job at world/character building. She was able to do so without interrupting the story which is one of my huge pet peeves. A lot of authors build by taking breaks in the plot or dialogue which I find choppy and disengaging but this author was able to build in an engaging way. There was some lack of world/character building but I think that was due to keeping reader in the dark. The world, politics, and characters were all very easy to understand.
I found the romance a little lackluster and a bit off-kilter. Some of it was really pushy and rushed which left a bad taste in my mouth. I do hope the author delves deeper into the romance in coming books.
The FMC was a breath of fresh air. She was not whiny or stuck-up like most books. She was never afraid to accept help from others but she was still able to shine with her intelligence. Not once did I think she made a poor decision just for the sake of a plot point. She was really easy to like. The MMC could use some work but I think that will happen more in coming books.
This is a MUST read, my only advice would be to wait until it is a finished series!
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Reviewed in the United States on July 30, 2025
★★★★★ 5
Need book 2 please :-)
Format: Kindle
From the very first chapter, Metal Slinger hooked me and refused to let go.
Set in a brutal, metal-bound world where power is carved from steel and survival depends on strength, strategy, and sheer will, this story follows a young woman forced into a dangerous system where control over metal isn’t just magic — it’s dominance, protection, and rebellion. When she’s pulled into escalating conflict and shifting alliances, she quickly learns that power always comes with a cost.
The magic system is sharp and immersive without being overwhelming. The way metal manipulation is woven into combat and politics makes every confrontation feel cinematic and high-stakes. It’s gritty, tense, and beautifully layered.
The Brynn, our FMC absolutely shines. She’s fierce but not untouchable, resilient without feeling unrealistic. Watching her navigate betrayal, survival, and the slow awakening of her own strength felt earned every step of the way. There’s grit in her fight scenes and vulnerability in her quieter moments that make her deeply human.
And the tension? Immaculate. The chemistry simmers under the surface before sparking into something electric. Every reluctant alliance, every charged interaction, every moment of “are they about to destroy each other or kiss?” had me flying through pages.
The pacing is relentless — no filler, no wasted scenes. Twists land hard. Stakes keep climbing. And that ending? Absolutely feral. The kind that leaves you staring at the wall for a minute before immediately checking when book two releases.
✨ Gritty metal-based magic
⚔️ High-stakes survival
🔥 Slow-burn tension
🖤 Fierce, layered heroine
💥 A finale that HITS (but cliffhanger!)
This is one of those fantasies that reminds you why you fell in love with the genre in the first place. If this is just Book One, I am more than ready to be emotionally wrecked again in the sequel.
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Reviewed in the United States on February 14, 2026
★★★★★ 5
Read this book! Then join our support group for the ending😜
While I’ve read some amazing books this year, it’s been awhile since I have completely devoured a book in less than 24 hours. This book was one I truly could not put down. I couldn’t fall asleep because I didn’t want to stop reading what happened next.
The way this book starts is one of my absolute favorite ways to fall into a story - with no preamble and immediate conspiring amongst characters.
Brynn has quickly become one of my new favorite FMCs. She is just so REAL. No whining or blaming her situation on everyone and everything else. She accepts her role in the world, but also holds a fierce determination to know the truth of that world. She is brave, yet terrified. Strong, yet self-conscious. She is cunning and intelligent and she doesn’t shy away from what is hard or allow emotion to overcome logic. I feel like she’s such a relatable character in everything that happens to her.
Then we have Kai…our commander and son of the leader. He’s got secrets. Big ones. And the amount of times my feelings for him flipped in this book is insane. I’m still honestly reeling…
Acker, my man…he’s mysterious, yet he doesn’t pull his punches. He’s loyal to a fault. He feels things deeply and he is fervent in his beliefs and desires. I adore him and yet want to shake him for the way he goes about making decisions and only sharing half truths. And right now my heart is aching for him and also terrified of him. When I say I need book 2 like I need water, that’s an understatement.
I don’t have space to discuss every side character, but just know that I love them deeply and they each have big personalities of their own.
The miscommunication honestly feels more like a necessity and less of a trope. The MCs don’t truly know one another, so of course there’s hesitancy to share everything with a stranger who has been somewhat forced upon you due to the situation you’ve both found yourself in. But it also is refreshing to watch them both learn to trust and communicate more and more as they go.
The world building was exciting and unique - unlike anything I’ve read before. The kingdoms and their systems and the unique magics all create this setting that is utterly perfect. I never felt confused, but could easily envision everything as it was explained in detail.
The slow burn and spice was paced so perfectly and realistically that it never felt weird or forced or annoying. It unfolds the way most normal relationships would (minus the magic and fantasy aspects). I truly cheered for our MCs and loved the way they finally came together.
This story takes all the tropes we know and love and twists them into something unique and entirely different than all the other stories we’ve grown used to. And when I tell you that you will NEVER see the plot twists coming, HOLY SMOKES BATMAN!
And the last 2 chapters took me ALL THE WAY OUT. I’m not sure how I’m supposed to be okay after that. I almost want to go back to chapter 55 and pretend the rest didn’t happen…but of course I can’t do that and now I’ll just be dying a little inside every day until I get my hands on book two!
If you enjoy fantasy or romantasy, this has to be your next read. All the rest can wait. Grab your copy YESTERDAY.
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Reviewed in the United States on June 24, 2025